Page 25 - Omics

Analysis Process
RAW Data
Xcms:
https://xcmsonline.scripps.edu/
MetaboAnalyst:
mzCloud:
https://www.mzcloud.org/ Qualitative metabolites https://www.metaboanalyst.ca/home.xh
tml
Analysis Platform
FastQC
Quality Control
Partial Least
Squares
Discrimination MultiQC
Analysis
Metabolites of multivariate statistical analysis
PLS-DA
screening criteria (VIP> 1) and univariate
statistical analysis screening criteria (FC> 2.0 and VIP Metabolite screening PCA Prcomp(R)
P value <0.05) are considered as metabolites with OPLS-DA
Heatmap Pheatmap(R)
significant differences
Orthogonal PLS-DA
Vocano ggplot2(R)
MetaboDiff(R)
Differential metabolites
MetaboAnalystR(R) CorrPlot Corrplot(R)

Z-score Plot Base stats(R)
KEGG Barplot
FELLA(R)
Enrichment
KEGG Dotplot Analysis Network Cytoscape

Result Report