Page 6 - Omics
P. 6

Experimental design
                                                                                WES-Seq (Whole Exome Sequencing)










                            1.
                   Samples-Tissues

                                                                                                                           Enrichment of DNA sequences in
                                                                                                                           exon regions using probe

                                                                                                                           hybridization
                                                             2.                                           3.
                                                           DNA                                        Library
                                                                                                                           Region 180bp~280bp
                           Total >= 0.4 μg
                                                                                                                           Agilent SureSelect Platform












                                                                                                                      4.
                                                                      Illumina PE150                          Illumina PE150
                                                                      Q30>=80%                                                                                          5.
                                                                                                                                                                 Bioinformatics
                                                                                                                                                                    Analysis
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