Page 11 - Omics
P. 11

Raw Reads
      Analysis Process


                                                                                                                        FastQC /
                                                                                            Quality Control         MultiQC(Python)



                                                                      Trimmomatic            Clean Reads               Ref Seq



                                                                          Hisat2 /
                                                                          Bowtie2           Mapped Reads



                                                                                              Transcript


                       Vocano Graph:         DESeq2(R) /
                         ggplot2(R)           edger(R)        Gene expression                                    New gene identification

                                                                                                                                                GATK4
                                                            Sample relationship
                                                                  analysis                                          Genetic structure
                                                                                                                      optimization



                                                             Differential gene
                                                                  analysis                                         Mutation analysis




                                                                                                                        RNA Edit

                      StringDB      PPI analysis            Enrichment analysis        clusterProfiler(R)
                                                                                                                                             STARfusion
                                                                                                                  Fusion gene analysis


                                              GO          KEGG          Reactome
                                                                                                                                               rMATS
                                                                                                                 Variable shear analysis
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