Page 10 - Omics

Experimental design Eukaryotic reference transcriptome

1. Clipping
Samples 2. mRNA:
Tissues 5’ UnTranslated Region, (UTR)

3’ Seq AAUAAA, (polyA)

5.
PolyT Enrichment
Extracting

4.
Remove rRNA

3.
RNA
(mRNA, rRNA, tRNA, ncRNA) rRNA is 82% in all RNA.

1.mRNA: Message RNA 6.
mRNA
2.tRNA: Translate RNA

3.rRNA: Ribosomal RNA
4.ncRNA: No Code RNA