Page 14 - Omics
P. 14
Experimental design
1.
Microbial community
1.
Microbial community
Concentration>=5ng/μL
Total>=150ng
Total>=200ng
2.
2. DNA
DNA
3.
PCR amplify 16S
Add adapter 3. DNA Library
DNA Library 16S Seq Metagenomics
1.DNA Fragments
2.Add adapter to two ends
3.350bp library
4.
Sequences 4.
Ion S5 XL Platform Sequences
NovaSeq 6000 Platform
Illumina Sequences platform
PE250/SE400 PE150
5. 5.
Bioinformatics Analysis
Bioinformatics Analysis
