Page 19 - Omics
P. 19

Experimental design







                      1.
                  Samples


                                                                                                                                                                      7.

                                                                                                                                                              Bioinformatics
                                                   2.
                                         Protein extraction                                                                                                       Analysis

                                                                             iTRAQ:Isobaric Tags for Relative and Absolute Quantitation
                                                                             TMT:Tandem Mass Tags






                                                                                                                    5.
                                                                                                             iTRAQ/TMT                                      6.
                                                          3.                                                                                          LC-MS/MS
                                                     Reductive
                                               alkylation(还原烷基化)
                                                                                                                                        1.LC-MS/MS:
                                                                                                                                        Liquid Chromatography - Mass
                                                                                                                                        Spectrometry

                                                                                                                                        2.TOF: Time of Flight
                4.                                                                             4.                                       3. ESI: Electron Spray Ionization

         Proteomics                                                                      Proteolysis                                    4. MALDI:
                                                                                                                                        Matrix-assisted Laser
                                                                                                                                        Desorption/ionization
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